东莞壬枚影视文化发展公司

您好,歡迎進(jìn)入研域(上海)化學(xué)試劑有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
  • 技術(shù)文章ARTICLE

    您當(dāng)前的位置:首頁 > 技術(shù)文章 > Plant Phosphatidic acid ELISA kit

    Plant Phosphatidic acid ELISA kit

    發(fā)布時(shí)間: 2010-04-19  點(diǎn)擊次數(shù): 1826次

     

    INTENDED USE
    The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
     
    PRINCIPLE OF THE METHOD
    The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
     
    REAGENTS PROVIDED AND RECONSTTTUTION

    REAGENTS(Store at 2-8℃)
    1×96 WELLS
    0.5×96 WELLS
    RECONSTTTUTION
    96/48-wells microtiter plates
    1
    0.5
    Ready-to-use
    Plastiv cover
    2
    1
    Ready-to-use
    Standard: 40ng/ml
    1Vials (0.6ml)
    0.5Vials (0.3ml)
    See reagents preparation on page 3
    Blank control
    1Vials (1.0ml)
    1Vials  (0.5ml)
    Ready-to-use
    Standard Diluent
    1Vials (5ml)
    1Vials   (2.5ml)
    Ready-to-use
    Biotinylated anti-PA
    1Vials (6ml)
    1Vials   (3.0ml)
    Ready-to-use
    Streptavidin-HRP
    1Vials (10ml)
    1Vials   (5.0ml)
    Ready-to-use
    Washing Buffer
    1Vials (60ml)
    1Vials   (30ml)
    20× concentrate
    Substrate  A
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Substrate  B
    1Vials 6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Stopping Solution
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use

     
    MATERIAL REQUIRED BUT NOT PROVIDED
    l       Distilled water
    l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
    l       Vortex mixer and magnetic stirrer.
     
    INTENDED USE
    The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
     
    PRINCIPLE OF THE METHOD
    The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
     
    REAGENTS PROVIDED AND RECONSTTTUTION

    REAGENTS(Store at 2-8℃)
    1×96 WELLS
    0.5×96 WELLS
    RECONSTTTUTION
    96/48-wells microtiter plates
    1
    0.5
    Ready-to-use
    Plastiv cover
    2
    1
    Ready-to-use
    Standard: 40ng/ml
    1Vials (0.6ml)
    0.5Vials (0.3ml)
    See reagents preparation on page 3
    Blank control
    1Vials (1.0ml)
    1Vials  (0.5ml)
    Ready-to-use
    Standard Diluent
    1Vials (5ml)
    1Vials   (2.5ml)
    Ready-to-use
    Biotinylated anti-PA
    1Vials (6ml)
    1Vials   (3.0ml)
    Ready-to-use
    Streptavidin-HRP
    1Vials (10ml)
    1Vials   (5.0ml)
    Ready-to-use
    Washing Buffer
    1Vials (60ml)
    1Vials   (30ml)
    20× concentrate
    Substrate  A
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Substrate  B
    1Vials 6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Stopping Solution
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use

     
    MATERIAL REQUIRED BUT NOT PROVIDED
    l       Distilled water
    l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
    l       Vortex mixer and magnetic stirrer.
     
     
產(chǎn)品中心 Products
在線客服 聯(lián)系方式

服務(wù)熱線

021-54479081
021-54461587

定远县| 柞水县| 桐柏县| 林周县| 泽普县| 呼图壁县| 南投市| 五家渠市| 江油市| 嘉鱼县| 浑源县| 亚东县| 响水县| 黔南| 五大连池市| 莱芜市| 桃园市| 牡丹江市| 达州市| 洪湖市| 齐河县| 苍溪县| 广东省| 公安县| 万安县| 苏州市| 调兵山市| 济阳县| 登封市| 永定县| 巍山| 林西县| 东兰县| 芜湖市| 平罗县| 铁力市| 仪陇县| 留坝县| 葫芦岛市| 永仁县| 手游|